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KMID : 1025520080500050641
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Journal of Animal Science and Technology
2008 Volume.50 No. 5 p.641 ~ p.648
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Identification of Differentially Expressed Genes Between Somatic Cell Nuclear Transfer and Normal Placenta in Cattle
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Yu Seong-Lan
Jeong Hang-Jin
Sang Byung-Chan
Ryoo Seung-Heui
Jung Kie-Chul
Yoon Jong-Taek
Seong Hwan-Hoo
Jin Dong-Il
Lee Jun-Heon
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Abstract
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There has been great success for making transgenic animals using somatic cell nuclear transfer(SCNT) up to this time. However, the success rates of the production of live transgenic animals are still very low. The current research has been carried out for delineation of differentially expressed genes between SCNT and normal placenta in cattle. In the present observations, high expression has been observed for CTSZ, LOC509426 and ELF1 genes in normal placenta. On the other hand, TIMP2, PAG1B, PAG-21, LOC782894, SERPINB6 and mKIAA2025 protein were highly expressed in SCNT placenta. Five genes, which were highly expressed in SCNT placenta, have been further investigated using semi-quantitative real-time PCR. The results were similar to that we observed using ACP. In the future, all genes affecting the SCNT and normal placenta have to be discovered and their networks will be fully investigated. The genes were identified in this study would be great help for identifying differential gene expressions in SCNT placenta.
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KEYWORD
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Somatic cell nuclear transfer, SCNT, placenta, Annealing control primer, ACP, Differentially expressed genes, DEGs
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